Validate your antibodies to improve reproducibility? Easier said than done

It seems like the most elementary of research principles: Make sure the cells and reagents in your experiment are what they claim to be and behave as expected. But when it comes to antibodies—the immune proteins used in all kinds of experiments to tag a molecule of interest in a sample—that validation process is not straightforward. Research antibodies from commercial vendors are often screened and optimized for narrow experimental conditions, which means they may not work as advertised for many scientists. Indeed, problems with antibodies are thought to have led many drug developers astray and generated a host of misleading or irreproducible scientific results.

A Reproducibility Study of Information Retrieval Models

Developing effective information retrieval models has been a long standing challenge in Information Retrieval (IR), and significant progresses have been made over the years. With the increasing number of developed retrieval functions and the release of new data collections, it becomes more difficult, if not impossible, to compare a new retrieval function with all existing retrieval functions over all available data collections. To tackle thisproblem, this paper describes our efforts on constructing a platform that aims to improve the reproducibility of IR researchand facilitate the evaluation and comparison of retrieval functions.

Reproducibility of Search Strategies Is Poor in Systematic Reviews Published in High-Impact Pediatrics, Cardiology and Surgery Journals: A Cross-Sectional Study

A high-quality search strategy is considered an essential component of systematic reviews but many do not contain reproducible search strategies. It is unclear if low reproducibility spans medical disciplines, is affected by librarian/search specialist involvement or has improved with increased awareness of reporting guidelines.

What do we mean by "reproducibility"?

There’s been a lot of discussion across many scientific fields about the "reproducibility crisis" in the past few years. Hundreds of psychologists attempted to redo 100 studies as part of the Reproducibility Project in Psychology, and claimed that fewer than half of the replication attempts succeeded. In Biomedicine, a study from the biotech firm Amgen tried to re-create results of 53 "landmark" preclinical cancer studies, and only got the same results for six of them. Amid a growing concern about research reliability, funders including the National Institutes of Health (NIH) have called for a greater effort to make research reproducible through transparent reporting of the methods researchers use to conduct their investigations.

Research Antibody Reproducibility

The ongoing dialogue has included the role of improperly validated research reagents, such as antibodies, with blame falling at the feet of reagent vendors, researchers, and journals. This article will highlight how the lack of consistent research on antibody validation has contributed to the reproducibility crisis and the role of vendors from Cell Signaling Technology’s (CST) perspective in making research more robust and reproducible.